The Forgotten Immunoglobulin LigaTrap™ Technologies and Advancements in IgM Purification
It's been over 30 years since the identification and commercialization of protein A. Since its inception protein A has served as the pinnacle purification platform for the immunoglobulin G (IgG) subclass of antibodies, both monoclonal and polyclonal forms. The introduction of protein A paved the way for researchers and large pharmaceutical manufacturers to develop and commercialize IgG-based therapeutics at both small and large scales, provided by its relative ease of use and well-characterized chromatographic attributes. Unfortunately, the gold standad affinity ligand, protein A, does not bind to all immunoglobulin subclasses, including immunoglobulin M (IgM0, thereby forcing those working with IgM to find alternative purification strategies and leaving IgM to hitchhike on that "yellow brick road" that protein A offers so readily for IgG.
Click here to read the complete white paper pdf and learn why the LigaTrapTM IgM purification resin has a clear advantage over the competition.