IgM Purification Kit and Resin

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 IgM Purification Using LigaTrap's® IgM Purification Resin

 

About IgM

IgM monoclonal antibodies have become increasingly favorable for their use as therapeutic tools, while remaining popular for their research and diagnostic applications. Protein A and G chromatography continues to lead the life science industries for the purification of IgG, however Protein A/G proves unsuitable for IgM purification proves. In addition to increased yields and purity levels, LigaTrap® Human IgM Purification Resin (LT-143) address many of the shortfalls related to standard affinity ligands including challenges with proteolysis and repeated sanitization associated with other common purification methodologies.

 

IgM Purification Resin and Kits

Part Number

Name

Amount

LT-143Kit

LigaTrap® Human IgM Purification Kit

1 Kit

LT-143

LigaTrap® Human IgM Purification Resin

5 ml

 

 

 

 IgM Purification Kit

 

IgM Yield and Purity

Human IgM was purified from CHO media containing 5% FBS using and a leading competitor of IgM purification resin. Standard chromatographic conditions were used for the LigaTrap® LT-143 resin. Three different manufacturer recommended binding conditions were used for the leading competitor of IgM purification resin.

 

 Purification of Human IgM 

 

 SDS PAGE of IgM Purification

 


 

About LigaTrap® Technologies

LigaTrap® purification kits and resins are convenient and easy-to-use. Simply add the sample to the resin, mix and centrifuge. Not only are LigaTrap® purification protocols simple to follow, but also allow for fast and easy recovery of purified antibodies for screening in your end-use assay. Multiple samples can be processed at the same time, resulting in rapid screening for antibody production. LigaTrap® spin columns only require a pipette and centrifuge, no specialized equipment needed. Each spin column is capable of up to 10 purifications, and each kit has the ability to perform over 100 purifications, making LigaTrap® a  cost-effective method for antibody purification.

  

About ImmunoReagents Inc.

ImmunoReagents, Inc. is a leading global manufacturer of quality antibodies and reagents used in pharmaceutical research, life science research and in vitro diagnostics. Product offerings include a wide range of immunochemistry reagents such as purified immunoglobulinsprimary antibodies, and secondary antibodies covering a broad spectrum of immunoglobulins from various species. ImmunoReagents also provides custom manufacturing to meet specific customer requirements while adhering to cGMP guidelines and ISO quality systems requirements. The company is located in Raleigh, North Carolina